Coding

Part:BBa_K5142035:Design

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-09-30)


Vaccinia virus A28L


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 463
    Illegal PstI site found at 477
    Illegal NotI site found at 7
    Illegal NotI site found at 470
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 463
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 463
    Illegal PstI site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, the A28L DNA sequence was served as a homologous arm for recombination only. Thus, the A28L sequence was not necessarily to be complete cds. However, considering the potential usage of A28L in the future or by others, we included the complete cds of A28L in this part.


Source

The cds of A28L was cloned by PCR amplification from the genome of vaccinia virus (GenBank ID: JX489136.1).

References